解剖学和形态学
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行为科学
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细胞和组织工程学
临床神经病学
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牙科,口腔外科和医学
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abstract::Tempe was prepared using mixtures of natural soybean and genetically modified Roundup Ready (RUR) soybean fermented with natural Rhizopus sp. The amount of RUR soybean was quantified using an ELISA plate test. The RUR signal decreased during fermentation. In the control experiments on fermentation of non-RUR soybean, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025449902523
更新日期:2003-09-01 00:00:00
abstract::The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix o...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025464918453
更新日期:2003-09-01 00:00:00
abstract::Kluyveromyces marxianus was grown in submerged culture in a complex medium with several potential inducers of lipolytic activity (triacylglycerols, fatty acids). The highest extracellular lipolytic enzyme production (about 80 U ml(-1) in 3 d) was obtained when the medium was supplemented with 2 g urea l(-1) plus 5 g t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025049825720
更新日期:2003-09-01 00:00:00
abstract::Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidas...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025072020791
更新日期:2003-09-01 00:00:00
abstract::The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respective...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024951322955
更新日期:2003-08-01 00:00:00
abstract::A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025016221891
更新日期:2003-08-01 00:00:00
abstract::Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (fro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025003800414
更新日期:2003-08-01 00:00:00
abstract::L-Lactic acid was produced from raw cassava starch, by simultaneous enzyme production, starch saccharification and fermentation in a circulating loop bioreactor with Aspergillus awamori and Lactococcus lactis spp. lactis immobilized in loofa sponge. A. awamori was immobilized directly in cylindrical loofa sponge while...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024192131343
更新日期:2003-07-01 00:00:00
abstract::Of several types of chemical surfactants and biosurfactants, only the culture broth of Bacillus subtilis C1 containing surfactin at 10 mg l(-1) completely inhibited the growth of Microcystis aeruginosa, a bloom-forming cyanobacterium in highly eutrophic lakes. The broth with 10 mg surfactin l(-1) also removed 85% of t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024508927361
更新日期:2003-07-01 00:00:00
abstract::Decrystallized chitosan was produced from shrimp shells with a low degree of crystallinity (10%) and a high anionic dye binding capacity. Raw, mixed dye wastewater from a textile factory was efficiently decolorized using decrystallized chitosan that was more efficient than using normal chitosan and activated carbon. D...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024562900548
更新日期:2003-07-01 00:00:00
abstract::We assessed the whole genome amplification strategy, known as multiple displacement amplification (MDA), for use with the TaqMan genotyping platform for DNA samples derived from two case-control studies nested in the Nurses' Health Study and the Physicians' Health Study. Our objectives were to (1) quantify DNA yield f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024173909401
更新日期:2003-07-01 00:00:00
abstract::Crude rapeseed oil and post-refining fatty acids were used as substrates for oxalic acid production by a mutant of Aspergillus niger. Both the final concentration and the yield of the product were highest at pH 4 to 5. With a medium containing 50 g lipids l(-1), production reached a maximum of 68 g oxalic acid l(-1) a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024082130677
更新日期:2003-06-01 00:00:00
abstract::Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024028603089
更新日期:2003-06-01 00:00:00
abstract::In batch culture for glutathione production with Candida utilis, a higher temperature (30 degrees C) was required to hasten cell growth while a lower temperature (26 degrees C) was needed to increase the production of glutathione. A two-stage temperature control strategy was used to enhance both the yield and the prod...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024034508136
更新日期:2003-06-01 00:00:00
abstract::Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023584805911
更新日期:2003-05-01 00:00:00
abstract::Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023402529882
更新日期:2003-05-01 00:00:00
abstract::Fourteen yeast strains were screened for production of 2-phenylethanol from L-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l-1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-pheny...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022890119847
更新日期:2003-04-01 00:00:00
abstract::Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytube...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023038804556
更新日期:2003-04-01 00:00:00
abstract::Trichodesmium sp., isolated from the Great Barrier Reef lagoon, was cultured in artificial seawater media containing a range of Fe concentration. Fe additions stimulated growth, N2 fixation, cellular chlorophyll a content, light-saturated chlorophyll a-specific gross photosynthetic capacity (Pmchl a) and the dark resp...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023068232375
更新日期:2003-04-01 00:00:00
abstract::A new cDNA (Os beta fruct2) encoding an acid beta-D-fructofuranosidase from rice has been cloned, sequenced and expressed in Pichia pastoris. The full-length cDNA is 2453 base pairs long and encodes a pre-pro-protein of 682 amino acids. The cDNA fragment coding for mature enzyme was sub-cloned into vector pPICZalphaA ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022648211997
更新日期:2003-03-01 00:00:00
abstract::A chip was developed to store DNA for medical research. The optional restriction site fixed on the chip can randomly ligate with whole human genomic DNA treated by the corresponding restriction enzyme. PCR can then use the chip as template DNA. Moreover, a chip fixing two restriction sites (e.g. EcoRI and HindIII) sho...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022626627231
更新日期:2003-03-01 00:00:00
abstract::The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022498314354
更新日期:2003-03-01 00:00:00
abstract::A novel method to examine the morphology and structure of fungal hyphae in solid pastes used for the production of meat alternative product is presented. A sample of fermentation broth was fluorescently stained with Calcofluor White and added back to the broth, mixed and then a paste made using ultra-filtration. Fibre...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022393116596
更新日期:2003-02-01 00:00:00
abstract::An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a relatively long distance between the transducer and reflector, were optimized. A tilted settler was design...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022345508979
更新日期:2003-02-01 00:00:00
abstract::To understand the mechanism of phosphate accumulation, a gene encoding polyphosphate kinase (PPK) was cloned from the genomic library of Serratia marcescens by Southern hybridization. From the nucleotide sequence of a 4 kb DNA fragment, an open reading frame of 2063 nucleotides was identified encoding a protein of 686...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022386514487
更新日期:2003-02-01 00:00:00
abstract::An unstructured model based on mass balance equations for a recombinant methylotrophic yeast Pichia pastoris MutS (Methanol Utilization Slow) strain expressing the mini-proinsulin (MPI), was successfully established in quasi-steady state fed-batch fermentations with varying total quantity of biomass in a 7 l fermenter...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022398917213
更新日期:2003-02-01 00:00:00
abstract::Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021919404066
更新日期:2003-01-01 00:00:00
abstract::Amberlite XAD 2 resin enhanced the asymmetric reduction of ethyl 4-chloroacetoacetate (ECA) to S-4-chloro-3-hydroxybutyric acid ethyl ester as catalyzed by Saccharomyces cerevisiae. The absorbed ECA was released slowly to the solution during the reaction so that the substrate inhibition and the spontaneous chemical hy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021761627090
更新日期:2003-01-01 00:00:00
abstract::Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021750719337
更新日期:2003-01-01 00:00:00
abstract::A mutant ofS. chrysomallus with a D-glucose isomerase inducible by D-glucose or its catabolites was characterized. In contrast to the wild-type strain it showed a decreased catabolite repression by D-glucose of D-xylose consumption and a constitutive pentose phosphate pathway as well. A hypothesis concerning altered i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/BF01026199
更新日期:1987-12-01 00:00:00